Real-time Multiplex Pcr Assay for Investigation of the Kinesin Light-chain 1 Gene A185c and C406t Mutations
نویسندگان
چکیده
The kinesin is the main motor protein in the trafficking system of the mitochondria and other organelles. Genetic variants affecting the function of kinesin were earlier found to be associated with cognitive disturbances and neurodegeneration. A multiplex polymerase chain reaction (mPCR) method, allowing the simultaneous detection of the KNS2 A185C (rs_1054080) and C406T (rs_3742465) mutations has been developed. The new assay was validated by PCR-RFLP experiments: the proposed method and the PCR-RFLP analysis yielded identical results. This suggests the applicability of the mPCR assay for the investigation of KNS2 mutations in population studies.
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